\name{ForkBatch} \alias{ForkBatch} \alias{OneBatch} \alias{OneBatch,RolexaRun-method} \title{Multi-threaded Probabilistic Base Calling} \description{Performs multi-threaded base calling on a collection of intensity files generated by the Solexa image analysis software} \usage{ ForkBatch(run=Rolexa.env,path,outpath="./",prefix="rs_",nthreads=3,nfiles=2,lane=1,tiles=1:100,...) \S4method{OneBatch}{RolexaRun}(run,path,lane,tiles,outpath,prefix) OneBatch(run,...) } \arguments{ \item{run}{a \link[=RolexaRun-class]{RolexaRun} object defining the run parameters} \item{path}{a \link[ShortRead:SolexaPath-class]{SolexaPath} object defining providing the input paths} \item{outpath}{the path to the output directory} \item{prefix}{output file prefix, see \code{\link{SaveResults}}} \item{nthreads}{number of threads to use} \item{nfiles}{number of input files to concatenate in one batch} \item{lane}{the lane number to analyze} \item{tiles}{a subset of tiles to read} \item{\dots}{further arguments passed to the \link[=RolexaRun-class]{RolexaRun} constructor} } \details{ The function \code{\link{ForkBatch}} runs through the list of input files, concatenates them by batches of \code{nfiles}, then calls \code{\link{OneBatch}} in each of the \code{nthreads} threads until all batches have been processed. Each batch results are passed to \code{\link{FilterResults}} and saved in an output file inside \code{outpath}. } \references{Probabilistic base calling of Solexa sequencing data, BMC Bioinformatics 2008, 9:431} \author{Jacques Rougemont, Arnaud Amzallag, Christian Iseli, Laurent Farinelli, Ioannis Xenarios, Felix Naef} \seealso{\code{\link{CombineFastQ}}, \code{\link{CombineReads}} and \code{\link{SaveResults}}} \examples{ path = SolexaPath(system.file("extdata", package="ShortRead")) rolenv = SetModel(idsep="_") \dontrun{ #This will take some time to complete: library(fork) ForkBatch(run=rolenv,path=path,tiles=1) } } \keyword{iteration} \keyword{utilities}