## ----setup, include = FALSE---------------------------------------------------
knitr::opts_chunk$set(
  collapse = TRUE,
  comment = "#>",
  fig.width = 8,
  fig.height = 6
)

# Load required packages
library(TmCalculator)
library(BSgenome.Hsapiens.UCSC.hg38)
library(GenomicRanges)
library(IRanges)

## ----install, eval = FALSE----------------------------------------------------
# # Install required packages
# suppressMessages({
#   library(BiocManager)
#   BiocManager::install(c("Biostrings", "BSgenome", "BSgenome.Hsapiens.UCSC.hg38"))
# })
# install.packages("TmCalculator")
# pak::pkg_install("Gviz")
# pak::pkg_install("JunhuiLi1017/TmCalculator@dev")

## ----basic_usage, message=FALSE-----------------------------------------------
# Calculate Tm for simple sequences
seqs <- c("ATGCGCGAAAT", "GCTAGCTAGCT")
names(seqs) <- c("chr1:1-11:+:seq1", "chr2:1-11:+:seq2")
result <- tm_calculate(seqs, method = "tm_wallace")
print(result)

## ----genomic_input, message=FALSE---------------------------------------------
# Example with genomic coordinates
coords <- c(
  "chr1:1898000-1898050:+:BSgenome.Hsapiens.UCSC.hg38:seq1",
  "chr2:2563000-2563050:-:BSgenome.Hsapiens.UCSC.hg38:seq2"
)
result <- tm_calculate(coords)
result

## ----genomic_input_options, message=FALSE-------------------------------------
result$tm$Options

## ----fasta_processing, message=FALSE------------------------------------------
# Process sequences from a FASTA file
fasta_file <- system.file("extdata", "example1.fasta", package = "TmCalculator")
result_fa <- tm_calculate(fasta_file)
result_fa

## ----complement_mismatch, message=FALSE---------------------------------------
# Example with provided complementary sequences
seqs <- c("GCTAGCCGACAATGGGCAGATAGTAGAAA")
comp_seqs <- c("CGATCGGCTATTACCCGTCTATCATCTTT")
result <- tm_calculate(input_seq=seqs,complement_seq=comp_seqs, method="tm_nn")
result

## ----complement_dangling_end, message=FALSE-----------------------------------
seqs <- c("GCTAGCCGACAATGGGCAGATAGTAGAAA")
comp_seqs <- c("GATCGGCTATTACCCGTCTATCATCTTT")
result <- tm_calculate(input_seq=seqs,
                       complement_seq=comp_seqs,
                       method="tm_nn",
                       shift=-1)
result

## ----tm_viz, message=FALSE----------------------------------------------------
# Generate sample data with 150 sequences
set.seed(123)

# Generate 100 sequences for chr1
chr1_starts <- sort(sample(1:249250621, 100))  # chr1 length in hg19
chr1_lengths <- sample(50:200, 100, replace=TRUE)
chr1_ends <- chr1_starts + chr1_lengths
chr1_tms <- runif(100, min=60, max=80)

# Generate 50 sequences for chr2
chr2_starts <- sort(sample(1:243199373, 50))   # chr2 length in hg19
chr2_lengths <- sample(50:200, 50, replace=TRUE)
chr2_ends <- chr2_starts + chr2_lengths
chr2_tms <- runif(50, min=60, max=80)

# Create GRanges object
tm_results <- GRanges(
  seqnames = Rle(c(rep("chr1", 100), rep("chr2", 50))),
  ranges = IRanges(
    start = c(chr1_starts, chr2_starts),
    end = c(chr1_ends, chr2_ends)
  ),
  strand = Rle(sample(c("+", "-"), 150, replace=TRUE)),
  Tm = c(chr1_tms, chr2_tms)
)
genome(seqinfo(tm_results)) <- "hg38"

# Example usage by plot_tm_karyotype
plot_tm_karyotype(tm_results, genome_assembly = "hg38")

# Example usage by plot_tm_genome_tracks with custom settings (plot_type=4)
plot_tm_karyotype(tm_results,
                  genome_assembly = "hg38",
                  plot_type = 4,
                  xaxis_cex = 0.5
                  )

# Example usage by plot_tm_genome_tracks with default settings (plot_type=1)
plot_tm_genome_tracks(tm_results, chromosome_to_plot = "chr1", genome_assembly = "hg38")

# Create example GRanges object  
gr_tm <- GenomicRanges::GRanges(
  seqnames = c("chr1", "chr2", "chr1", "chr2", "chr1"),
  ranges = IRanges::IRanges(
    start = c(100, 200, 300, 400, 150),
    end = c(150, 250, 350, 450, 200)
  ),
  Tm = c(65.5, 68.2, 70.1, 63.8, 72.0)
)

# Plot with ideograms
plot_tm_heatmap(gr_tm, genome_assembly = "hg19", plot_type = "karyogram")

# Faceted plot by chromosome
plot_tm_heatmap(gr_tm, genome_assembly = "hg19", plot_type = "faceted")

## ----launch_shiny, eval = FALSE-----------------------------------------------
# TmCalculatorShiny::TmCalculator_shiny()