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This page was generated on 2024-04-18 11:32:18 -0400 (Thu, 18 Apr 2024).
Hostname | OS | Arch (*) | R version | Installed pkgs |
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kjohnson1 | macOS 13.6.1 Ventura | arm64 | 4.3.3 (2024-02-29) -- "Angel Food Cake" | 4388 |
Click on any hostname to see more info about the system (e.g. compilers) (*) as reported by 'uname -p', except on Windows and Mac OS X |
Package 1838/2266 | Hostname | OS / Arch | INSTALL | BUILD | CHECK | BUILD BIN | ||||||||
sangeranalyseR 1.12.0 (landing page) Kuan-Hao Chao
| kjohnson1 | macOS 13.6.1 Ventura / arm64 | OK | OK | WARNINGS | OK | ||||||||
To the developers/maintainers of the sangeranalyseR package: - Use the following Renviron settings to reproduce errors and warnings. - If 'R CMD check' started to fail recently on the Linux builder(s) over a missing dependency, add the missing dependency to 'Suggests:' in your DESCRIPTION file. See Renviron.bioc for more information. |
Package: sangeranalyseR |
Version: 1.12.0 |
Command: /Library/Frameworks/R.framework/Resources/bin/R CMD check --install=check:sangeranalyseR.install-out.txt --library=/Library/Frameworks/R.framework/Resources/library --no-vignettes --timings sangeranalyseR_1.12.0.tar.gz |
StartedAt: 2024-04-18 02:24:48 -0400 (Thu, 18 Apr 2024) |
EndedAt: 2024-04-18 02:30:54 -0400 (Thu, 18 Apr 2024) |
EllapsedTime: 366.3 seconds |
RetCode: 0 |
Status: WARNINGS |
CheckDir: sangeranalyseR.Rcheck |
Warnings: 7 |
############################################################################## ############################################################################## ### ### Running command: ### ### /Library/Frameworks/R.framework/Resources/bin/R CMD check --install=check:sangeranalyseR.install-out.txt --library=/Library/Frameworks/R.framework/Resources/library --no-vignettes --timings sangeranalyseR_1.12.0.tar.gz ### ############################################################################## ############################################################################## * using log directory ‘/Users/biocbuild/bbs-3.18-bioc-mac-arm64/meat/sangeranalyseR.Rcheck’ * using R version 4.3.3 (2024-02-29) * using platform: aarch64-apple-darwin20 (64-bit) * R was compiled by Apple clang version 14.0.0 (clang-1400.0.29.202) GNU Fortran (GCC) 12.2.0 * running under: macOS Ventura 13.6.1 * using session charset: UTF-8 * using option ‘--no-vignettes’ * checking for file ‘sangeranalyseR/DESCRIPTION’ ... OK * checking extension type ... Package * this is package ‘sangeranalyseR’ version ‘1.12.0’ * package encoding: UTF-8 * checking package namespace information ... OK * checking package dependencies ... NOTE Depends: includes the non-default packages: 'stringr', 'ape', 'Biostrings', 'DECIPHER', 'parallel', 'reshape2', 'phangorn', 'sangerseqR', 'gridExtra', 'shiny', 'shinydashboard', 'shinyjs', 'data.table', 'plotly', 'DT', 'zeallot', 'excelR', 'shinycssloaders', 'ggdendro', 'shinyWidgets', 'openxlsx', 'tools', 'rmarkdown', 'knitr', 'seqinr', 'BiocStyle', 'logger' Adding so many packages to the search path is excessive and importing selectively is preferable. * checking if this is a source package ... OK * checking if there is a namespace ... OK * checking for hidden files and directories ... NOTE Found the following hidden files and directories: .travis.yml docs/build/html/.buildinfo These were most likely included in error. See section ‘Package structure’ in the ‘Writing R Extensions’ manual. * checking for portable file names ... WARNING Found the following file with a non-portable file name: docs/wallpaperflare.com_wallpaper (1).jpg These are not fully portable file names. See section ‘Package structure’ in the ‘Writing R Extensions’ manual. Found the following non-portable file paths: sangeranalyseR/docs/build/html/_downloads/081987116457968e1b81ae1616525c7d/Sanger_contigs_unalignment.fa sangeranalyseR/docs/build/html/_downloads/13b875bc704f73b8bd67dc07d86fc474/Sanger_contigs_alignment.fa sangeranalyseR/docs/build/html/_downloads/144d09562ae5b2c7dc079e4bc2b33fb7/Sanger_contigs_alignment.fa sangeranalyseR/docs/build/html/_downloads/165af37e8893cfdfa4dba91c44cd791b/Sanger_contigs_unalignment.fa sangeranalyseR/docs/build/html/_downloads/345ac400befc4f176c75732770ec3fcb/Achl_RBNII384-13_contig.fa sangeranalyseR/docs/build/html/_downloads/438c8bd47d93d92c65b35b613ef14739/Sanger_all_trimmed_reads.fa sangeranalyseR/docs/build/html/_downloads/6211ea1e7e3ccc1fcc9b74b78224eab6/Achl_ACHLO006-09_reads_unalignment.fa sangeranalyseR/docs/build/html/_downloads/67bcad64cca804ec2e14cc42d016c08f/Achl_ACHLO006-09_reads_alignment.fa sangeranalyseR/docs/build/html/_downloads/81a7338101f110748cb72056d9975225/Sanger_all_trimmed_reads.fa sangeranalyseR/docs/build/html/_downloads/95ef9378c1407481d69c3bf882a580bb/Achl_RBNII384-13_reads_unalignment.fa sangeranalyseR/docs/build/html/_downloads/a664bed9cc5d32924dc2ce60f8c60554/Sanger_all_trimmed_reads.fa sangeranalyseR/docs/build/html/_downloads/b608796fffaaa7335a7341cffc9a9db7/Achl_ACHLO006-09_contig.fa sangeranalyseR/docs/build/html/_downloads/b6b18aa3e34c6ae0be86c3940972dd38/Sanger_contigs_alignment.fa sangeranalyseR/docs/build/html/_downloads/eeb554b575ee5b29ef733dafbf876c36/Achl_RBNII384-13_reads_alignment.fa sangeranalyseR/docs/build/html/_downloads/fef71e7b2e1e49c01df301435e972047/Sanger_contigs_unalignment.fa Tarballs are only required to store paths of up to 100 bytes and cannot store those of more than 256 bytes, with restrictions including to 100 bytes for the final component. See section ‘Package structure’ in the ‘Writing R Extensions’ manual. * checking for sufficient/correct file permissions ... OK * checking whether package ‘sangeranalyseR’ can be installed ... OK * checking installed package size ... NOTE installed size is 8.5Mb sub-directories of 1Mb or more: data 2.2Mb extdata 5.5Mb * checking package directory ... OK * checking ‘build’ directory ... OK * checking DESCRIPTION meta-information ... OK * checking top-level files ... NOTE File LICENSE is not mentioned in the DESCRIPTION file. * checking for left-over files ... OK * checking index information ... OK * checking package subdirectories ... OK * checking R files for non-ASCII characters ... WARNING Found the following files with non-ASCII characters: ClassSangerAlignment.R ClassSangerContig.R ClassSangerRead.R Portable packages must use only ASCII characters in their R code, except perhaps in comments. Use \uxxxx escapes for other characters. * checking R files for syntax errors ... OK * checking whether the package can be loaded ... OK * checking whether the package can be loaded with stated dependencies ... OK * checking whether the package can be unloaded cleanly ... OK * checking whether the namespace can be loaded with stated dependencies ... OK * checking whether the namespace can be unloaded cleanly ... OK * checking startup messages can be suppressed ... OK * checking dependencies in R code ... NOTE Packages in Depends field not imported from: ‘BiocStyle’ ‘knitr’ ‘phangorn’ ‘reshape2’ ‘zeallot’ These packages need to be imported from (in the NAMESPACE file) for when this namespace is loaded but not attached. package 'methods' is used but not declared * checking S3 generic/method consistency ... OK * checking replacement functions ... OK * checking foreign function calls ... OK * checking R code for possible problems ... NOTE QualityBasePlotly: no visible global function definition for ‘%>%’ SangerAlignment: no visible global function definition for ‘new’ SangerAlignmentServer: no visible global function definition for ‘%>%’ SangerAlignmentServer: no visible binding for '<<-' assignment to ‘NEW_SANGER_ALIGNED_CONSENSUS_READ_SET’ SangerAlignmentServer: no visible global function definition for ‘colorRamp’ SangerContig: no visible global function definition for ‘new’ SangerContigServer: no visible global function definition for ‘%>%’ SangerContigServer: no visible binding for '<<-' assignment to ‘NEW_SANGER_CONTIG’ SangerContigServer: no visible global function definition for ‘colorRamp’ SangerRead: no visible global function definition for ‘new’ SetCharStyleList: no visible global function definition for ‘%>%’ alignContigs: no visible global function definition for ‘as.phylo’ alignContigs: no visible global function definition for ‘rtree’ checkAb1FastaCsv: no visible global function definition for ‘read.csv’ checkTargetFastaName: no visible global function definition for ‘isEmpty’ chromatogram_overwrite: no visible global function definition for ‘rgb’ chromatogram_overwrite: no visible global function definition for ‘par’ chromatogram_overwrite: no visible global function definition for ‘quantile’ chromatogram_overwrite: no visible global function definition for ‘IQR’ chromatogram_overwrite: no visible global function definition for ‘pdf’ chromatogram_overwrite: no visible global function definition for ‘rect’ chromatogram_overwrite: no visible global function definition for ‘lines’ chromatogram_overwrite: no visible global function definition for ‘mtext’ chromatogram_overwrite: no visible global function definition for ‘axis’ chromatogram_overwrite: no visible global function definition for ‘dev.off’ primarySeqDisplay: no visible global function definition for ‘rgb’ primarySeqTrimmedDisplay: no visible global function definition for ‘rgb’ secondSeqTrimmedDisplay: no visible global function definition for ‘rgb’ secondarySeqDisplay: no visible global function definition for ‘rgb’ initialize,ChromatogramParam: no visible global function definition for ‘callNextMethod’ initialize,ObjectResults: no visible global function definition for ‘callNextMethod’ initialize,QualityReport: no visible global function definition for ‘callNextMethod’ initialize,SangerAlignment: no visible global function definition for ‘str_split’ initialize,SangerAlignment : <anonymous>: no visible global function definition for ‘new’ initialize,SangerAlignment: no visible global function definition for ‘read.csv’ initialize,SangerAlignment: no visible global function definition for ‘new’ initialize,SangerAlignment: no visible global function definition for ‘callNextMethod’ initialize,SangerContig : <anonymous>: no visible global function definition for ‘new’ initialize,SangerContig: no visible global function definition for ‘read.csv’ initialize,SangerContig: no visible global function definition for ‘new’ initialize,SangerContig: no visible global function definition for ‘callNextMethod’ initialize,SangerRead: no visible global function definition for ‘new’ initialize,SangerRead: no visible global function definition for ‘AAString’ initialize,SangerRead: no visible global function definition for ‘callNextMethod’ launchAppSA,SangerAlignment: no visible global function definition for ‘shinyOptions’ launchAppSA,SangerAlignment: no visible global function definition for ‘shinyApp’ launchAppSC,SangerContig: no visible global function definition for ‘shinyOptions’ launchAppSC,SangerContig: no visible global function definition for ‘shinyApp’ Undefined global functions or variables: %>% AAString IQR as.phylo axis callNextMethod colorRamp dev.off isEmpty lines mtext new par pdf quantile read.csv rect rgb rtree shinyApp shinyOptions str_split Consider adding importFrom("grDevices", "colorRamp", "dev.off", "pdf", "rgb") importFrom("graphics", "axis", "lines", "mtext", "par", "rect") importFrom("methods", "callNextMethod", "new") importFrom("stats", "IQR", "quantile") importFrom("utils", "read.csv") to your NAMESPACE file (and ensure that your DESCRIPTION Imports field contains 'methods'). * checking Rd files ... OK * checking Rd metadata ... OK * checking Rd cross-references ... OK * checking for missing documentation entries ... WARNING Undocumented code objects: ‘chromatogram_overwrite’ All user-level objects in a package should have documentation entries. See chapter ‘Writing R documentation files’ in the ‘Writing R Extensions’ manual. * checking for code/documentation mismatches ... WARNING S4 class codoc mismatches from documentation object 'ObjectResults-class': Slots for class 'ObjectResults' Code: creationResult errorMessages errorTypes printLevel readResultTable warningMessages warningTypes Docs: printLevel * checking Rd \usage sections ... WARNING Undocumented arguments in documentation object 'SangerAlignment' ‘printLevel’ ‘processMethod’ Documented arguments not in \usage in documentation object 'SangerAlignment': ‘minFractionCallSA’ ‘maxFractionLostSA’ Undocumented arguments in documentation object 'SangerContig' ‘printLevel’ ‘processMethod’ Undocumented arguments in documentation object 'SangerRead' ‘printLevel’ Functions with \usage entries need to have the appropriate \alias entries, and all their arguments documented. The \usage entries must correspond to syntactically valid R code. See chapter ‘Writing R documentation files’ in the ‘Writing R Extensions’ manual. * checking Rd contents ... OK * checking for unstated dependencies in examples ... OK * checking contents of ‘data’ directory ... OK * checking data for non-ASCII characters ... WARNING Failed with error: 'package 'phangorn' could not be loaded' Error loading dataset 'qualityReportData': Error in .requirePackage(package) : unable to find required package 'sangeranalyseR' Failed with error: 'package 'phangorn' could not be loaded' Error loading dataset 'sangerAlignmentData': Error in .requirePackage(package) : unable to find required package 'sangeranalyseR' Failed with error: 'package 'phangorn' could not be loaded' Error loading dataset 'sangerContigData': Error in .requirePackage(package) : unable to find required package 'sangeranalyseR' Failed with error: 'package 'phangorn' could not be loaded' Error loading dataset 'sangerReadFData': Error in .requirePackage(package) : unable to find required package 'sangeranalyseR' The dataset(s) may use package(s) not declared in Depends/Imports. * checking data for ASCII and uncompressed saves ... WARNING Note: significantly better compression could be obtained by using R CMD build --resave-data old_size new_size compress sangerAlignmentData.RData 1.5Mb 699Kb xz sangerContigData.RData 451Kb 203Kb xz sangerReadFData.RData 269Kb 119Kb xz * checking line endings in Makefiles ... OK * checking for GNU extensions in Makefiles ... OK * checking include directives in Makefiles ... OK * checking files in ‘vignettes’ ... OK * checking examples ... OK Examples with CPU (user + system) or elapsed time > 5s user system elapsed SangerAlignment-class 51.122 1.507 52.798 SangerContig-class 10.221 0.310 10.547 SangerAlignment 10.042 0.335 10.449 * checking for unstated dependencies in ‘tests’ ... OK * checking tests ... Running ‘testthat.R’ OK * checking for unstated dependencies in vignettes ... OK * checking package vignettes in ‘inst/doc’ ... OK * checking running R code from vignettes ... SKIPPED * checking re-building of vignette outputs ... SKIPPED * checking PDF version of manual ... OK * DONE Status: 7 WARNINGs, 6 NOTEs See ‘/Users/biocbuild/bbs-3.18-bioc-mac-arm64/meat/sangeranalyseR.Rcheck/00check.log’ for details.
sangeranalyseR.Rcheck/00install.out
############################################################################## ############################################################################## ### ### Running command: ### ### /Library/Frameworks/R.framework/Resources/bin/R CMD INSTALL sangeranalyseR ### ############################################################################## ############################################################################## * installing to library ‘/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library’ * installing *source* package ‘sangeranalyseR’ ... ** using staged installation ** R ** data ** inst ** byte-compile and prepare package for lazy loading Note: wrong number of arguments to '==' ** help *** installing help indices ** building package indices ** installing vignettes ** testing if installed package can be loaded from temporary location ** testing if installed package can be loaded from final location ** testing if installed package keeps a record of temporary installation path * DONE (sangeranalyseR)
sangeranalyseR.Rcheck/tests/testthat.Rout
R version 4.3.3 (2024-02-29) -- "Angel Food Cake" Copyright (C) 2024 The R Foundation for Statistical Computing Platform: aarch64-apple-darwin20 (64-bit) R is free software and comes with ABSOLUTELY NO WARRANTY. You are welcome to redistribute it under certain conditions. Type 'license()' or 'licence()' for distribution details. R is a collaborative project with many contributors. Type 'contributors()' for more information and 'citation()' on how to cite R or R packages in publications. Type 'demo()' for some demos, 'help()' for on-line help, or 'help.start()' for an HTML browser interface to help. Type 'q()' to quit R. > library(testthat) > library(sangeranalyseR) Loading required package: stringr Loading required package: ape Loading required package: Biostrings Loading required package: BiocGenerics Attaching package: 'BiocGenerics' The following objects are masked from 'package:stats': IQR, mad, sd, var, xtabs The following objects are masked from 'package:base': Filter, Find, Map, Position, Reduce, anyDuplicated, aperm, append, as.data.frame, basename, cbind, colnames, dirname, do.call, duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted, lapply, mapply, match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank, rbind, rownames, sapply, setdiff, sort, table, tapply, union, unique, unsplit, which.max, which.min Loading required package: S4Vectors Loading required package: stats4 Attaching package: 'S4Vectors' The following object is masked from 'package:utils': findMatches The following objects are masked from 'package:base': I, expand.grid, unname Loading required package: IRanges Loading required package: XVector Loading required package: GenomeInfoDb Attaching package: 'Biostrings' The following object is masked from 'package:ape': complement The following object is masked from 'package:base': strsplit Loading required package: DECIPHER Loading required package: RSQLite Loading required package: parallel Loading required package: reshape2 Loading required package: phangorn Loading required package: sangerseqR Loading required package: gridExtra Attaching package: 'gridExtra' The following object is masked from 'package:BiocGenerics': combine Loading required package: shiny Loading required package: shinydashboard Attaching package: 'shinydashboard' The following object is masked from 'package:graphics': box Loading required package: shinyjs Attaching package: 'shinyjs' The following object is masked from 'package:shiny': runExample The following object is masked from 'package:RSQLite': show The following object is masked from 'package:Biostrings': show The following object is masked from 'package:GenomeInfoDb': show The following object is masked from 'package:XVector': show The following object is masked from 'package:IRanges': show The following object is masked from 'package:S4Vectors': show The following object is masked from 'package:stats4': show The following objects are masked from 'package:methods': removeClass, show Loading required package: data.table Attaching package: 'data.table' The following objects are masked from 'package:reshape2': dcast, melt The following object is masked from 'package:IRanges': shift The following objects are masked from 'package:S4Vectors': first, second Loading required package: plotly Loading required package: ggplot2 Attaching package: 'plotly' The following object is masked from 'package:ggplot2': last_plot The following object is masked from 'package:phangorn': add_boxplot The following object is masked from 'package:XVector': slice The following object is masked from 'package:IRanges': slice The following object is masked from 'package:S4Vectors': rename The following object is masked from 'package:stats': filter The following object is masked from 'package:graphics': layout Loading required package: DT Attaching package: 'DT' The following objects are masked from 'package:shiny': dataTableOutput, renderDataTable Loading required package: zeallot Loading required package: excelR Loading required package: shinycssloaders Loading required package: ggdendro Loading required package: shinyWidgets Attaching package: 'shinyWidgets' The following object is masked from 'package:shinyjs': alert Loading required package: openxlsx Loading required package: tools Loading required package: rmarkdown Loading required package: knitr Loading required package: seqinr Attaching package: 'seqinr' The following object is masked from 'package:shiny': a The following object is masked from 'package:sangerseqR': read.abif The following object is masked from 'package:Biostrings': translate The following objects are masked from 'package:ape': as.alignment, consensus Loading required package: BiocStyle Attaching package: 'BiocStyle' The following objects are masked from 'package:rmarkdown': html_document, md_document, pdf_document The following object is masked from 'package:shiny': markdown Loading required package: logger Welcome to sangeranalyseR > > > test_check("sangeranalyseR") INFO [2024-04-18 02:29:35] ################################################# INFO [2024-04-18 02:29:35] #### Start creating SangerAlignment instance #### INFO [2024-04-18 02:29:35] ################################################# INFO [2024-04-18 02:29:35] >> You are using Regular Expression Method to group AB1 files! INFO [2024-04-18 02:29:35] ======================================================== INFO [2024-04-18 02:29:35] ================ Creating 'SangerContig' =============== INFO [2024-04-18 02:29:35] ======================================================== INFO [2024-04-18 02:29:35] >> Contig Name: 'Achl_ACHLO006-09' SUCCESS [2024-04-18 02:29:36] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:36] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:29:36] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:36] >> 'Achl_ACHLO006-09_1_F.ab1' is created (Forward Read; ABIF). SUCCESS [2024-04-18 02:29:37] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:37] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:29:37] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:37] >> 'Achl_ACHLO006-09_2_R.ab1' is created (Reverse Read; ABIF). INFO [2024-04-18 02:29:37] >> The number of reads detected: 2 Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.06 secs SUCCESS [2024-04-18 02:29:39] ========================================================== SUCCESS [2024-04-18 02:29:39] ======== 'SangerContig' S4 instance is created !! ======== SUCCESS [2024-04-18 02:29:39] ========================================================== INFO [2024-04-18 02:29:39] >> 2 read(s) created from ABIF file. INFO [2024-04-18 02:29:39] >> 1 reads assigned to 'forward reads' according to 'regular expression'. INFO [2024-04-18 02:29:39] >> 1 reads assigned to 'reverse reads' according to 'regular expression'. INFO [2024-04-18 02:29:39] ======================================================== INFO [2024-04-18 02:29:39] ================ Creating 'SangerContig' =============== INFO [2024-04-18 02:29:39] ======================================================== INFO [2024-04-18 02:29:39] >> Contig Name: 'Achl_ACHLO007-09' SUCCESS [2024-04-18 02:29:40] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:40] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:29:40] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:40] >> 'Achl_ACHLO007-09_1_F.ab1' is created (Forward Read; ABIF). SUCCESS [2024-04-18 02:29:40] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:40] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:29:40] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:40] >> 'Achl_ACHLO007-09_2_R.ab1' is created (Reverse Read; ABIF). INFO [2024-04-18 02:29:40] >> The number of reads detected: 2 Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.04 secs SUCCESS [2024-04-18 02:29:42] ========================================================== SUCCESS [2024-04-18 02:29:42] ======== 'SangerContig' S4 instance is created !! ======== SUCCESS [2024-04-18 02:29:42] ========================================================== INFO [2024-04-18 02:29:42] >> 2 read(s) created from ABIF file. INFO [2024-04-18 02:29:42] >> 1 reads assigned to 'forward reads' according to 'regular expression'. INFO [2024-04-18 02:29:42] >> 1 reads assigned to 'reverse reads' according to 'regular expression'. INFO [2024-04-18 02:29:42] ======================================================== INFO [2024-04-18 02:29:42] ================ Creating 'SangerContig' =============== INFO [2024-04-18 02:29:42] ======================================================== INFO [2024-04-18 02:29:42] >> Contig Name: 'Achl_ACHLO040-09' SUCCESS [2024-04-18 02:29:42] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:42] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:29:42] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:42] >> 'Achl_ACHLO040-09_1_F.ab1' is created (Forward Read; ABIF). SUCCESS [2024-04-18 02:29:43] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:43] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:29:43] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:43] >> 'Achl_ACHLO040-09_2_R.ab1' is created (Reverse Read; ABIF). INFO [2024-04-18 02:29:43] >> The number of reads detected: 2 Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.04 secs SUCCESS [2024-04-18 02:29:44] ========================================================== SUCCESS [2024-04-18 02:29:44] ======== 'SangerContig' S4 instance is created !! ======== SUCCESS [2024-04-18 02:29:44] ========================================================== INFO [2024-04-18 02:29:44] >> 2 read(s) created from ABIF file. INFO [2024-04-18 02:29:44] >> 1 reads assigned to 'forward reads' according to 'regular expression'. INFO [2024-04-18 02:29:44] >> 1 reads assigned to 'reverse reads' according to 'regular expression'. INFO [2024-04-18 02:29:44] ======================================================== INFO [2024-04-18 02:29:44] ================ Creating 'SangerContig' =============== INFO [2024-04-18 02:29:44] ======================================================== INFO [2024-04-18 02:29:44] >> Contig Name: 'Achl_ACHLO041-09' SUCCESS [2024-04-18 02:29:45] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:45] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:29:45] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:45] >> 'Achl_ACHLO041-09_1_F.ab1' is created (Forward Read; ABIF). SUCCESS [2024-04-18 02:29:45] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:46] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:29:46] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:46] >> 'Achl_ACHLO041-09_2_R.ab1' is created (Reverse Read; ABIF). INFO [2024-04-18 02:29:46] >> The number of reads detected: 2 Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.04 secs SUCCESS [2024-04-18 02:29:47] ========================================================== SUCCESS [2024-04-18 02:29:47] ======== 'SangerContig' S4 instance is created !! ======== SUCCESS [2024-04-18 02:29:47] ========================================================== INFO [2024-04-18 02:29:47] >> 2 read(s) created from ABIF file. INFO [2024-04-18 02:29:47] >> 1 reads assigned to 'forward reads' according to 'regular expression'. INFO [2024-04-18 02:29:47] >> 1 reads assigned to 'reverse reads' according to 'regular expression'. INFO [2024-04-18 02:29:47] Aligning consensus reads ... SUCCESS [2024-04-18 02:29:48] ############################################################# SUCCESS [2024-04-18 02:29:48] ######## 'SangerAlignment' S4 instance is created !! ######## SUCCESS [2024-04-18 02:29:48] ############################################################# INFO [2024-04-18 02:29:48] >> 4 contigs detected from 'regular expression'. INFO [2024-04-18 02:29:48] >> Contig 'Achl_ACHLO006-09': INFO [2024-04-18 02:29:48] >> 1 forward reads. INFO [2024-04-18 02:29:48] >> 1 reverse reads. INFO [2024-04-18 02:29:48] >> Contig 'Achl_ACHLO007-09': INFO [2024-04-18 02:29:48] >> 1 forward reads. INFO [2024-04-18 02:29:48] >> 1 reverse reads. INFO [2024-04-18 02:29:48] >> Contig 'Achl_ACHLO040-09': INFO [2024-04-18 02:29:48] >> 1 forward reads. INFO [2024-04-18 02:29:48] >> 1 reverse reads. INFO [2024-04-18 02:29:48] >> Contig 'Achl_ACHLO041-09': INFO [2024-04-18 02:29:48] >> 1 forward reads. INFO [2024-04-18 02:29:48] >> 1 reverse reads. INFO [2024-04-18 02:29:48] >> 8 reads created from ABIF file. INFO [2024-04-18 02:29:48] >> Reads are trimmed by 'M1 - Mott’s trimming algorithm'. DEBUG [2024-04-18 02:29:48] >> For more information, please run 'object'. DEBUG [2024-04-18 02:29:48] >> Run 'object@objectResults@readResultTable' to check the results of each Sanger reads INFO [2024-04-18 02:29:49] ################################################# INFO [2024-04-18 02:29:49] #### Start creating SangerAlignment instance #### INFO [2024-04-18 02:29:49] ################################################# INFO [2024-04-18 02:29:49] **** You are using Regular Expression Method to group reads in FASTA file (No CSV file)! INFO [2024-04-18 02:29:49] ======================================================== INFO [2024-04-18 02:29:49] ================ Creating 'SangerContig' =============== INFO [2024-04-18 02:29:49] ======================================================== INFO [2024-04-18 02:29:49] >> Contig Name: 'Achl_ACHLO006-09' SUCCESS [2024-04-18 02:29:49] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:49] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:29:49] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:49] >> 'Achl_ACHLO006-09_1_F' is created (Forward Read; FASTA). SUCCESS [2024-04-18 02:29:49] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:49] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:29:49] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:49] >> 'Achl_ACHLO006-09_2_R' is created (Reverse Read; FASTA). INFO [2024-04-18 02:29:49] >> The number of reads detected: 2 Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.04 secs SUCCESS [2024-04-18 02:29:51] ========================================================== SUCCESS [2024-04-18 02:29:51] ======== 'SangerContig' S4 instance is created !! ======== SUCCESS [2024-04-18 02:29:51] ========================================================== INFO [2024-04-18 02:29:51] >> 2 read(s) created from FASTA file. INFO [2024-04-18 02:29:51] >> 1 reads assigned to 'forward reads' according to 'regular expression'. INFO [2024-04-18 02:29:51] >> 1 reads assigned to 'reverse reads' according to 'regular expression'. INFO [2024-04-18 02:29:51] ======================================================== INFO [2024-04-18 02:29:51] ================ Creating 'SangerContig' =============== INFO [2024-04-18 02:29:51] ======================================================== INFO [2024-04-18 02:29:51] >> Contig Name: 'Achl_ACHLO007-09' SUCCESS [2024-04-18 02:29:51] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:51] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:29:51] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:51] >> 'Achl_ACHLO007-09_1_F' is created (Forward Read; FASTA). SUCCESS [2024-04-18 02:29:51] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:51] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:29:51] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:51] >> 'Achl_ACHLO007-09_2_R' is created (Reverse Read; FASTA). INFO [2024-04-18 02:29:51] >> The number of reads detected: 2 Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.04 secs SUCCESS [2024-04-18 02:29:53] ========================================================== SUCCESS [2024-04-18 02:29:53] ======== 'SangerContig' S4 instance is created !! ======== SUCCESS [2024-04-18 02:29:53] ========================================================== INFO [2024-04-18 02:29:53] >> 2 read(s) created from FASTA file. INFO [2024-04-18 02:29:53] >> 1 reads assigned to 'forward reads' according to 'regular expression'. INFO [2024-04-18 02:29:53] >> 1 reads assigned to 'reverse reads' according to 'regular expression'. INFO [2024-04-18 02:29:53] ======================================================== INFO [2024-04-18 02:29:53] ================ Creating 'SangerContig' =============== INFO [2024-04-18 02:29:53] ======================================================== INFO [2024-04-18 02:29:53] >> Contig Name: 'Achl_ACHLO040-09' SUCCESS [2024-04-18 02:29:53] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:53] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:29:53] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:53] >> 'Achl_ACHLO040-09_1_F' is created (Forward Read; FASTA). SUCCESS [2024-04-18 02:29:53] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:53] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:29:53] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:53] >> 'Achl_ACHLO040-09_2_R' is created (Reverse Read; FASTA). INFO [2024-04-18 02:29:53] >> The number of reads detected: 2 Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.04 secs SUCCESS [2024-04-18 02:29:55] ========================================================== SUCCESS [2024-04-18 02:29:55] ======== 'SangerContig' S4 instance is created !! ======== SUCCESS [2024-04-18 02:29:55] ========================================================== INFO [2024-04-18 02:29:55] >> 2 read(s) created from FASTA file. INFO [2024-04-18 02:29:55] >> 1 reads assigned to 'forward reads' according to 'regular expression'. INFO [2024-04-18 02:29:55] >> 1 reads assigned to 'reverse reads' according to 'regular expression'. INFO [2024-04-18 02:29:55] ======================================================== INFO [2024-04-18 02:29:55] ================ Creating 'SangerContig' =============== INFO [2024-04-18 02:29:55] ======================================================== INFO [2024-04-18 02:29:55] >> Contig Name: 'Achl_ACHLO041-09' SUCCESS [2024-04-18 02:29:55] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:55] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:29:55] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:55] >> 'Achl_ACHLO041-09_1_F' is created (Forward Read; FASTA). SUCCESS [2024-04-18 02:29:55] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:55] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:29:55] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:55] >> 'Achl_ACHLO041-09_2_R' is created (Reverse Read; FASTA). INFO [2024-04-18 02:29:55] >> The number of reads detected: 2 Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.04 secs SUCCESS [2024-04-18 02:29:57] ========================================================== SUCCESS [2024-04-18 02:29:57] ======== 'SangerContig' S4 instance is created !! ======== SUCCESS [2024-04-18 02:29:57] ========================================================== INFO [2024-04-18 02:29:57] >> 2 read(s) created from FASTA file. INFO [2024-04-18 02:29:57] >> 1 reads assigned to 'forward reads' according to 'regular expression'. INFO [2024-04-18 02:29:57] >> 1 reads assigned to 'reverse reads' according to 'regular expression'. INFO [2024-04-18 02:29:57] Aligning consensus reads ... SUCCESS [2024-04-18 02:29:58] ############################################################# SUCCESS [2024-04-18 02:29:58] ######## 'SangerAlignment' S4 instance is created !! ######## SUCCESS [2024-04-18 02:29:58] ############################################################# INFO [2024-04-18 02:29:58] >> 4 contigs detected from 'regular expression'. INFO [2024-04-18 02:29:58] >> Contig 'Achl_ACHLO006-09': INFO [2024-04-18 02:29:58] >> 1 forward reads. INFO [2024-04-18 02:29:58] >> 1 reverse reads. INFO [2024-04-18 02:29:58] >> Contig 'Achl_ACHLO007-09': INFO [2024-04-18 02:29:58] >> 1 forward reads. INFO [2024-04-18 02:29:58] >> 1 reverse reads. INFO [2024-04-18 02:29:58] >> Contig 'Achl_ACHLO040-09': INFO [2024-04-18 02:29:58] >> 1 forward reads. INFO [2024-04-18 02:29:58] >> 1 reverse reads. INFO [2024-04-18 02:29:58] >> Contig 'Achl_ACHLO041-09': INFO [2024-04-18 02:29:58] >> 1 forward reads. INFO [2024-04-18 02:29:58] >> 1 reverse reads. INFO [2024-04-18 02:29:58] >> 8 reads created from FASTA file. DEBUG [2024-04-18 02:29:58] >> For more information, please run 'object'. DEBUG [2024-04-18 02:29:58] >> Run 'object@objectResults@readResultTable' to check the results of each Sanger reads INFO [2024-04-18 02:29:58] ======================================================== INFO [2024-04-18 02:29:58] ================ Creating 'SangerContig' =============== INFO [2024-04-18 02:29:58] ======================================================== INFO [2024-04-18 02:29:58] >> Contig Name: 'Achl_ACHLO006-09' INFO [2024-04-18 02:29:58] >> You are using Regular Expression Method to group AB1 files! INFO [2024-04-18 02:29:58] >> Your contig name is Achl_ACHLO006-09 SUCCESS [2024-04-18 02:29:58] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:58] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:29:58] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:58] >> 'Achl_ACHLO006-09_1_F.ab1' is created (Forward Read; ABIF). SUCCESS [2024-04-18 02:29:59] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:59] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:29:59] -------------------------------------------------------- SUCCESS [2024-04-18 02:29:59] >> 'Achl_ACHLO006-09_2_R.ab1' is created (Reverse Read; ABIF). INFO [2024-04-18 02:29:59] >> The number of reads detected: 2 INFO [2024-04-18 02:29:59] Correcting frameshifts in reads using amino acidreference sequence Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.04 secs SUCCESS [2024-04-18 02:30:00] ========================================================== SUCCESS [2024-04-18 02:30:00] ======== 'SangerContig' S4 instance is created !! ======== SUCCESS [2024-04-18 02:30:00] ========================================================== INFO [2024-04-18 02:30:00] >> 2 read(s) created from ABIF file. INFO [2024-04-18 02:30:00] >> 1 reads assigned to 'forward reads' according to 'regular expression'. INFO [2024-04-18 02:30:00] >> 1 reads assigned to 'reverse reads' according to 'regular expression'. INFO [2024-04-18 02:30:00] >> Trimmed by 'M1 - Mott’s trimming algorithm'. DEBUG [2024-04-18 02:30:00] >> For more information, please run 'object' DEBUG [2024-04-18 02:30:00] >> Run 'object@objectResults@readResultTable' to check the results of each Sanger reads INFO [2024-04-18 02:30:00] ======================================================== INFO [2024-04-18 02:30:00] ================ Creating 'SangerContig' =============== INFO [2024-04-18 02:30:00] ======================================================== INFO [2024-04-18 02:30:00] >> Contig Name: 'Achl_RBNII384-13' INFO [2024-04-18 02:30:00] >> You are using Regular Expression Method to group AB1 files! INFO [2024-04-18 02:30:00] >> Your contig name is Achl_RBNII384-13 SUCCESS [2024-04-18 02:30:01] -------------------------------------------------------- SUCCESS [2024-04-18 02:30:01] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:30:01] -------------------------------------------------------- SUCCESS [2024-04-18 02:30:01] >> 'Achl_RBNII384-13_1_F.ab1' is created (Forward Read; ABIF). SUCCESS [2024-04-18 02:30:01] -------------------------------------------------------- SUCCESS [2024-04-18 02:30:01] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:30:01] -------------------------------------------------------- SUCCESS [2024-04-18 02:30:01] >> 'Achl_RBNII384-13_2_R.ab1' is created (Reverse Read; ABIF). INFO [2024-04-18 02:30:01] >> The number of reads detected: 2 INFO [2024-04-18 02:30:01] Correcting frameshifts in reads using amino acidreference sequence Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.04 secs SUCCESS [2024-04-18 02:30:03] ========================================================== SUCCESS [2024-04-18 02:30:03] ======== 'SangerContig' S4 instance is created !! ======== SUCCESS [2024-04-18 02:30:03] ========================================================== INFO [2024-04-18 02:30:03] >> 2 read(s) created from ABIF file. INFO [2024-04-18 02:30:03] >> 1 reads assigned to 'forward reads' according to 'regular expression'. INFO [2024-04-18 02:30:03] >> 1 reads assigned to 'reverse reads' according to 'regular expression'. INFO [2024-04-18 02:30:03] >> Trimmed by 'M1 - Mott’s trimming algorithm'. DEBUG [2024-04-18 02:30:03] >> For more information, please run 'object' DEBUG [2024-04-18 02:30:03] >> Run 'object@objectResults@readResultTable' to check the results of each Sanger reads INFO [2024-04-18 02:30:03] ======================================================== INFO [2024-04-18 02:30:03] ================ Creating 'SangerContig' =============== INFO [2024-04-18 02:30:03] ======================================================== INFO [2024-04-18 02:30:03] >> Contig Name: 'Achl_ACHLO006-09' INFO [2024-04-18 02:30:03] >> You are using Regular Expression Method to group reads in FASTA file (No CSV file)! INFO [2024-04-18 02:30:03] >> Your contig name is Achl_ACHLO006-09 SUCCESS [2024-04-18 02:30:03] -------------------------------------------------------- SUCCESS [2024-04-18 02:30:03] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:30:03] -------------------------------------------------------- SUCCESS [2024-04-18 02:30:03] >> 'Achl_ACHLO006-09_1_F' is created (Forward Read; FASTA). SUCCESS [2024-04-18 02:30:03] -------------------------------------------------------- SUCCESS [2024-04-18 02:30:03] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:30:03] -------------------------------------------------------- SUCCESS [2024-04-18 02:30:03] >> 'Achl_ACHLO006-09_2_R' is created (Reverse Read; FASTA). INFO [2024-04-18 02:30:03] >> The number of reads detected: 2 INFO [2024-04-18 02:30:03] Correcting frameshifts in reads using amino acidreference sequence Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.04 secs SUCCESS [2024-04-18 02:30:05] ========================================================== SUCCESS [2024-04-18 02:30:05] ======== 'SangerContig' S4 instance is created !! ======== SUCCESS [2024-04-18 02:30:05] ========================================================== INFO [2024-04-18 02:30:05] >> 2 read(s) created from FASTA file. INFO [2024-04-18 02:30:05] >> 1 reads assigned to 'forward reads' according to 'regular expression'. INFO [2024-04-18 02:30:05] >> 1 reads assigned to 'reverse reads' according to 'regular expression'. DEBUG [2024-04-18 02:30:05] >> For more information, please run 'object' DEBUG [2024-04-18 02:30:05] >> Run 'object@objectResults@readResultTable' to check the results of each Sanger reads INFO [2024-04-18 02:30:05] ======================================================== INFO [2024-04-18 02:30:05] ================ Creating 'SangerContig' =============== INFO [2024-04-18 02:30:05] ======================================================== INFO [2024-04-18 02:30:05] >> Contig Name: 'Achl_RBNII384-13' INFO [2024-04-18 02:30:05] >> You are using CSV Name Conversion Method to group reads in FASTA file (with Csv file)! INFO [2024-04-18 02:30:05] >> Your contig name is Achl_RBNII384-13 SUCCESS [2024-04-18 02:30:05] -------------------------------------------------------- SUCCESS [2024-04-18 02:30:05] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:30:05] -------------------------------------------------------- SUCCESS [2024-04-18 02:30:05] >> 'Achl_RBNII384-13_1_F' is created (Forward Read; FASTA). SUCCESS [2024-04-18 02:30:05] -------------------------------------------------------- SUCCESS [2024-04-18 02:30:06] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:30:06] -------------------------------------------------------- SUCCESS [2024-04-18 02:30:06] >> 'Achl_RBNII384-13_2_R' is created (Reverse Read; FASTA). INFO [2024-04-18 02:30:06] >> The number of reads detected: 2 INFO [2024-04-18 02:30:06] Correcting frameshifts in reads using amino acidreference sequence Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.04 secs SUCCESS [2024-04-18 02:30:07] ========================================================== SUCCESS [2024-04-18 02:30:07] ======== 'SangerContig' S4 instance is created !! ======== SUCCESS [2024-04-18 02:30:07] ========================================================== INFO [2024-04-18 02:30:07] >> 2 read(s) created from FASTA file. INFO [2024-04-18 02:30:07] >> 1 reads assigned to 'forward reads' according to 'csv file'. INFO [2024-04-18 02:30:07] >> 1 reads assigned to 'reverse reads' according to 'csv file'. DEBUG [2024-04-18 02:30:07] >> For more information, please run 'object' DEBUG [2024-04-18 02:30:07] >> Run 'object@objectResults@readResultTable' to check the results of each Sanger reads INFO [2024-04-18 02:30:07] ------------------------------------------------ INFO [2024-04-18 02:30:07] -------- Creating 'SangerRead' instance -------- INFO [2024-04-18 02:30:07] ------------------------------------------------ INFO [2024-04-18 02:30:07] >> Forward Read: Creating abif & sangerseq ... INFO [2024-04-18 02:30:07] >> Creating Forward Read raw abif ... INFO [2024-04-18 02:30:07] >> Creating Forward Read raw sangerseq ... INFO [2024-04-18 02:30:07] * Making basecall !! INFO [2024-04-18 02:30:07] * Updating slots in 'SangerRead' instance !! SUCCESS [2024-04-18 02:30:08] -------------------------------------------------------- SUCCESS [2024-04-18 02:30:08] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:30:08] -------------------------------------------------------- SUCCESS [2024-04-18 02:30:08] >> 'Achl_ACHLO006-09_1_F.ab1' is created (Forward Read; ABIF). INFO [2024-04-18 02:30:08] >> Read is trimmed by 'M1 - Mott’s trimming algorithm'. DEBUG [2024-04-18 02:30:08] >> For more information, please run 'object'. DEBUG [2024-04-18 02:30:08] >> Run 'object@objectResults@readResultTable' to check the result of the Sanger read INFO [2024-04-18 02:30:08] ------------------------------------------------ INFO [2024-04-18 02:30:08] -------- Creating 'SangerRead' instance -------- INFO [2024-04-18 02:30:08] ------------------------------------------------ INFO [2024-04-18 02:30:08] >> Reverse Read: Creating abif & sangerseq ... INFO [2024-04-18 02:30:08] >> Creating Reverse Read raw abif ... INFO [2024-04-18 02:30:08] >> Creating Reverse Read raw sangerseq ... INFO [2024-04-18 02:30:08] * Making basecall !! INFO [2024-04-18 02:30:08] * Updating slots in 'SangerRead' instance !! SUCCESS [2024-04-18 02:30:08] -------------------------------------------------------- SUCCESS [2024-04-18 02:30:08] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:30:08] -------------------------------------------------------- SUCCESS [2024-04-18 02:30:08] >> 'Achl_ACHLO006-09_2_R.ab1' is created (Reverse Read; ABIF). INFO [2024-04-18 02:30:08] >> Read is trimmed by 'M1 - Mott’s trimming algorithm'. DEBUG [2024-04-18 02:30:08] >> For more information, please run 'object'. DEBUG [2024-04-18 02:30:08] >> Run 'object@objectResults@readResultTable' to check the result of the Sanger read INFO [2024-04-18 02:30:08] ------------------------------------------------ INFO [2024-04-18 02:30:08] -------- Creating 'SangerRead' instance -------- INFO [2024-04-18 02:30:08] ------------------------------------------------ INFO [2024-04-18 02:30:08] Forward Read: Creating SangerRead from FASTA ... SUCCESS [2024-04-18 02:30:08] -------------------------------------------------------- SUCCESS [2024-04-18 02:30:09] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:30:09] -------------------------------------------------------- SUCCESS [2024-04-18 02:30:09] >> 'Achl_ACHLO006-09_1_F' is created (Forward Read; FASTA). INFO [2024-04-18 02:30:09] >> Read is trimmed by 'M1 - Mott’s trimming algorithm'. DEBUG [2024-04-18 02:30:09] >> For more information, please run 'object'. DEBUG [2024-04-18 02:30:09] >> Run 'object@objectResults@readResultTable' to check the result of the Sanger read INFO [2024-04-18 02:30:09] ------------------------------------------------ INFO [2024-04-18 02:30:09] -------- Creating 'SangerRead' instance -------- INFO [2024-04-18 02:30:09] ------------------------------------------------ INFO [2024-04-18 02:30:09] Reverse Read: Creating SangerRead from FASTA ... SUCCESS [2024-04-18 02:30:09] -------------------------------------------------------- SUCCESS [2024-04-18 02:30:09] -------- 'SangerRead' S4 instance is created !! -------- SUCCESS [2024-04-18 02:30:09] -------------------------------------------------------- SUCCESS [2024-04-18 02:30:09] >> 'Achl_ACHLO006-09_2_R' is created (Reverse Read; FASTA). INFO [2024-04-18 02:30:09] >> Read is trimmed by 'M1 - Mott’s trimming algorithm'. DEBUG [2024-04-18 02:30:09] >> For more information, please run 'object'. DEBUG [2024-04-18 02:30:09] >> Run 'object@objectResults@readResultTable' to check the result of the Sanger read ERROR [2024-04-18 02:30:09] FILE_NOT_EXIST_ERROR '/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/sangeranalyseR/extdata//Allolobophora_chlorotica/ACHLO/TEST_Achl_ACHLO006-09_2_R.ab1' file does not exist. DEBUG [2024-04-18 02:30:09] >> For more information, please run 'object'. DEBUG [2024-04-18 02:30:09] >> Run 'object@objectResults@readResultTable' to check the result of the Sanger read Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.04 secs Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.04 secs Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.04 secs Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.04 secs INFO [2024-04-18 02:30:17] Aligning consensus reads ... Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.04 secs Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.04 secs Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.04 secs Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.04 secs INFO [2024-04-18 02:30:26] Aligning consensus reads ... Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.04 secs Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.04 secs INFO [2024-04-18 02:30:32] * Making basecall !! INFO [2024-04-18 02:30:33] * Updating slots in 'SangerRead' instance !! INFO [2024-04-18 02:30:33] * Making basecall !! INFO [2024-04-18 02:30:33] * Updating slots in 'SangerRead' instance !! INFO [2024-04-18 02:30:34] * Making basecall !! INFO [2024-04-18 02:30:34] * Updating slots in 'SangerRead' instance !! INFO [2024-04-18 02:30:34] * Making basecall !! INFO [2024-04-18 02:30:34] * Updating slots in 'SangerRead' instance !! INFO [2024-04-18 02:30:35] * Making basecall !! INFO [2024-04-18 02:30:35] * Updating slots in 'SangerRead' instance !! INFO [2024-04-18 02:30:38] >>> outputDir : /private/tmp/RtmpUk8BHr INFO [2024-04-18 02:30:38] Start writing '/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/sangeranalyseR/extdata//Allolobophora_chlorotica/ACHLO/Achl_ACHLO006-09_1_F.ab1' to FASTA format ... INFO [2024-04-18 02:30:38] >> '/private/tmp/RtmpUk8BHr/Achl_ACHLO006-09_1_F.fa' is written INFO [2024-04-18 02:30:38] >>> outputDir : /private/tmp/RtmpUk8BHr INFO [2024-04-18 02:30:38] Start writing '/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/sangeranalyseR/extdata//fasta/SangerRead/Achl_ACHLO006-09_1_F.fa' to FASTA format ... INFO [2024-04-18 02:30:38] >> '/private/tmp/RtmpUk8BHr/Achl_ACHLO006-09_1_F.fa' is written INFO [2024-04-18 02:30:38] >>> outputDir : /private/tmp/RtmpUk8BHr INFO [2024-04-18 02:30:38] Start writing '/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/sangeranalyseR/extdata//fasta/SangerRead/Achl_ACHLO006-09_2_R.fa' to FASTA format ... INFO [2024-04-18 02:30:38] >> '/private/tmp/RtmpUk8BHr/Achl_ACHLO006-09_2_R.fa' is written INFO [2024-04-18 02:30:38] Aligning consensus reads ... Assessing frameshifts in nucleotide sequences: ================================================================================ Time difference of 0.04 secs INFO [2024-04-18 02:30:40] * Making basecall !! INFO [2024-04-18 02:30:40] * Updating slots in 'SangerRead' instance !! INFO [2024-04-18 02:30:40] * Making basecall !! INFO [2024-04-18 02:30:41] * Updating slots in 'SangerRead' instance !! [ FAIL 0 | WARN 0 | SKIP 0 | PASS 899 ] > > proc.time() user system elapsed 72.294 1.845 74.288
sangeranalyseR.Rcheck/sangeranalyseR-Ex.timings
name | user | system | elapsed | |
ChromatogramParam-class | 0.006 | 0.001 | 0.006 | |
MakeBaseCalls-methods | 1.190 | 0.041 | 1.233 | |
ObjectResults-class | 0.003 | 0.001 | 0.003 | |
QualityReport-class-qualityBasePlot | 0.002 | 0.005 | 0.007 | |
QualityReport-class-updateQualityParam | 0.007 | 0.013 | 0.021 | |
QualityReport-class | 0.581 | 0.041 | 0.624 | |
SangerAlignment-class-generateReportSA | 0.103 | 0.008 | 0.111 | |
SangerAlignment-class-launchAppSA | 0.109 | 0.008 | 0.117 | |
SangerAlignment-class-updateQualityParam | 0.088 | 0.007 | 0.095 | |
SangerAlignment-class-writeFastaSA | 0.121 | 0.011 | 0.132 | |
SangerAlignment-class | 51.122 | 1.507 | 52.798 | |
SangerAlignment | 10.042 | 0.335 | 10.449 | |
SangerContig-class-generateReportSC | 0.046 | 0.005 | 0.050 | |
SangerContig-class-launchAppSC | 0.062 | 0.006 | 0.068 | |
SangerContig-class-readTable | 0.171 | 0.017 | 0.189 | |
SangerContig-class-updateQualityParam | 0.046 | 0.006 | 0.051 | |
SangerContig-class-writeFastaSC | 0.085 | 0.009 | 0.094 | |
SangerContig-class | 10.221 | 0.310 | 10.547 | |
SangerContig | 0.036 | 0.003 | 0.039 | |
SangerRead-class-MakeBaseCalls | 0.481 | 0.030 | 0.511 | |
SangerRead-class-generateReportSR | 0.038 | 0.005 | 0.043 | |
SangerRead-class-qualityBasePlot | 0.038 | 0.005 | 0.043 | |
SangerRead-class-readTable | 0.170 | 0.017 | 0.186 | |
SangerRead-class-updateQualityParam | 0.296 | 0.009 | 0.305 | |
SangerRead-class-writeFastaSR | 0.053 | 0.006 | 0.059 | |
SangerRead-class | 2.202 | 0.104 | 2.308 | |
SangerRead | 0.575 | 0.031 | 0.606 | |
generateReport | 0.171 | 0.018 | 0.190 | |
generateReportSA-methods | 0.088 | 0.006 | 0.095 | |
generateReportSC-methods | 0.045 | 0.005 | 0.051 | |
generateReportSR-methods | 0.038 | 0.005 | 0.044 | |
launchApp | 0.134 | 0.013 | 0.146 | |
launchAppSA-methods | 0.088 | 0.006 | 0.093 | |
launchAppSC-methods | 0.045 | 0.005 | 0.050 | |
qualityBasePlot-methods | 0.427 | 0.040 | 0.467 | |
readTable-methods | 0.161 | 0.016 | 0.179 | |
updateQualityParam-methods | 0.193 | 0.023 | 0.221 | |
writeFasta | 0.173 | 0.016 | 0.189 | |
writeFastaSA-methods | 0.110 | 0.009 | 0.119 | |
writeFastaSC-methods | 0.081 | 0.008 | 0.088 | |
writeFastaSR-methods | 0.052 | 0.006 | 0.058 | |